Recombinant GPNMB Monoclonal Antibody (AN301540L)
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For research use only.
| Verified Samples |
Verified Samples in WB: A375, B16-F0, EL4.IL-2, HeLa Verified Samples in IHC: Human tonsil, Mouse small intestine Verified Samples in FCM: A375, EL4 |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:1000, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human GPNMB fragment |
| Abbre | GPNMB |
| Synonyms | UNQ, PRO, GPNMB, HGFIN, NMB, Glycoprotein nmb, Glycoprotein nmb like protein, Osteoactivin, Transmembrane glycoprotein, Transmembrane glycoprotein HGFIN, Transmembrane glycoprotein NMB, UNQ1725, PRO9925, Glycoprotein nmb like protein, Gpnmb, HGFIN, NMB, Osteoactivin, Transmembrane glycoprotein, Transmembrane glycoprotein HGFIN, Transmembrane glycoprotein NMB |
| Swissprot | |
| Calculated MW | 64 kDa |
| Observed MW |
120,95,65 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Endosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cancer |
| Clone No. | A239 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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