Recombinant GRP75 Monoclonal Antibody (AN301691L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, MCF-7 Verified Samples in IF: MCF7 |
| Dilution | WB 1:500-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human GRP75 fragment |
| Abbre | GRP75 |
| Synonyms | CRP, PBP, HSPA, MTHSP, SIDBA, mt-HSP, GRP, HSPA9, CRP40, CSA, EVPLS, GRP-75, GRP75, HEL-S-124m, HSPA9B, MOT, MOT2, MTHSP75, PBP74, SAAN, SIDBA4, mt-HSP70, 75 kDa glucose regulated protein, 75 kDa glucose-regulated protein, 75 kDa glucose-regulated protein (GRP-75), Glucose Regulated Protein, Grp 75, Heat shock 70 kDa protein 9, Heat shock 70kD protein 9, heat shock 70kDa protein 9, Heat shock 70kDa protein 9B, Heat shock protein 74 kDa A, Heat shock protein A, Heat shock protein cognate 74, Hsc74, Hsp74, Hsp74a, Hspa9a, MGC4500, mitochondrial, Mortalin, Mortalin 2, Mortalin perinuclear, Mortalin2, MOT 2, Mthsp70, p66 mortalin, P66 MOT, PBP74, HSP70, Peptide binding protein 74, Peptide-binding protein 74, Peptide-binding protein 74 (PBP74), Stress 70 protein mitochondrial, Stress 70 protein mitochondrial precursor, Stress-70 protein |
| Swissprot | |
| Calculated MW | 74 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion, Nucleus, Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction, Cancer, Metabolism |
| Clone No. | A394 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging. |
Other Clones
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Unconjugated
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