Recombinant GSTK1 Monoclonal Antibody (AN301766L)
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For research use only.
| Verified Samples |
Verified Samples in WB: A431, HeLa, SK-BR-3 Verified Samples in IHC: Human kidney Verified Samples in IF: A549 Verified Samples in IP: A431 cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:100-1:500, IF 1:50, IP 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human GSTK1 fragment |
| Abbre | GSTK1 |
| Synonyms | GSTK, glutathione S-transferase kappa, hGSTK, GST 13, GSTK1, GST, GST 13-13, GST13, GST13-13, GSTK1-1, hGSTK1, glutathione S-transferase kappa 1, HDCMD47P, glutathione S-transferase kappa 1, GST, GST 13-13, GST13, GST13-13, GSTK1-1, hGSTK1, EC 2.5.1.18, Glutathione S transferase subunit 13, Glutathione S-transferase k1, Glutathione S-transferase subunit 13, Glutathione S-transferase subunit 13 homolog, GST 13 13, GST class kappa, GST class-kappa, GSTK1 1, hglutathione S-transferase kappa 1 |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
25 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Peroxisome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cell Biology, Metabolism |
| Clone No. | A474 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This protein is a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. |
Other Clones
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Other Formats
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Unconjugated
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