Recombinant GTPase HRAS Monoclonal Antibody (E-AB-81564)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, CHO-K1, C6 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Hamster |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human Ras |
| Abbre | GTPase HRAS |
| Synonyms | ALPS4, AV095280, GTPase NRas, HRAS1, N ras, N ras protein part 4, NRAS, NRAS1, NS6, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHUMP00000013879, OTTMUSP00000023521, RASN, Transforming protein N Ras, Transforming protein N-Ras, v ras neuroblastoma RAS viral onco |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
21 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Signal Transduction |
| Clone No. | R01-7D0 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane.This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex.The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. |
Other Clones
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Other Formats
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Unconjugated
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