Recombinant HMGA1 Monoclonal Antibody (AN301734L)
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For research use only.
| Verified Samples |
Verified Samples in IHC: Mouse testis, Human bladder cancer, Human testis Verified Samples in IF: MCF7 Verified Samples in WB: A549, NCCIT |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human HMGA1 fragment |
| Abbre | HMGA1 |
| Synonyms | HMGA, HMGA1, HMG-R, HMGA1A, HMGIY, High mobility group (nonhistone chromosomal) protein isoforms I and Y, High mobility group AT hook 1, High mobility group AT-hook protein 1, High mobility group protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein Ia, High mobility group protein R, HMG R, HMG-I(Y), MGC12816, MGC4242, MGC4854, Nonhistone chromosomal high mobility group protein HMG I/HMG Y |
| Swissprot | |
| Calculated MW | 12 kDa |
| Observed MW |
16 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Microbiology, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | A442 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | HMGA1 a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. HMGA1 preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. |
Other Clones
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Other Formats
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Unconjugated
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