Recombinant HMGB1 Monoclonal Antibody (AN301554L)

Name: Recombinant HMGB1 Monoclonal Antibody
Brand: Elabscience
SKU: AN301554L
Price: 249.0 USD
Availability: InStock

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Recombinant HMGB1 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: HeLa, HepG2, Jurkat, NIH/3T3, PC-12 Verified Samples in IHC: Human kidney, Mouse stomach, Rat colon Verified Samples in FCM: HeLa Verified Samples in IP: Jurkat cells extracts
Dilution	WB 1:500-1:2000, IHC 1:200-1:1000, FCM 1:50-1:100, IP 1:25
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, FCM, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human HMGB1 fragment
Abbre	HMGB1
Synonyms	SBP, HMGB, High Mobility Group Protein, High mobility group protein B, HMG, HMG-1, HMG1, HMG3, SBP-1, HMGB1, Amphoterin, High Mobility Group Protein 1, High mobility group protein B1, High mobility group protein 1 (HMG-1), High mobility group protein 1, high mobility group protein B1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, HMGB1, SBP-1, Chromosomal protein, DKFZp686A04236, High mobility group 1, High mobility group box 1, high-mobility group (nonhistone chromosomal) protein 1, HMGB 1, nonhistone, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein
Swissprot	P09429
Calculated MW	25 kDa
Observed MW	25 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Nucleus
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Epigenetics and Nuclear Signaling
Clone No.	A253
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury.