Recombinant HMGB2 Monoclonal Antibody (AN301821L)

Name: Recombinant HMGB2 Monoclonal Antibody
Brand: Elabscience
SKU: AN301821L
Price: 249.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Recombinant HMGB2 Monoclonal Antibody - 1

All	Size	Price	Qty
	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Verified Samples	Verified Samples in WB: HeLa, HepG2, PC-12 Verified Samples in IHC: Human colon, Mouse testis, Rat cerebrum Verified Samples in IF: HeLa Verified Samples in IP: HeLa cells extracts
Dilution	WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50, IP 1:25-1:50
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, IF, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human HMGB2 fragment
Abbre	HMGB2
Synonyms	HMGB, High mobility group box, High mobility group (nonhistone chromosomal) protein, HMG B, HMG, HMGB2, HMG2, C80539, High mobility group (nonhistone chromosomal) protein 2, High mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG B2, HMG-2
Swissprot	P26583
Calculated MW	24 kDa
Observed MW	24 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytoplasm, Nucleus, secreted
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Epigenetics and Nuclear Signaling
Clone No.	A533
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	HMGB2 belongs to a family of highly conserved proteins that contain HMG box domains. HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals. HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters. Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes. In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation end products (RAGE).