Recombinant HMGCR Monoclonal Antibody (AN301555L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, HepG2 Verified Samples in IF: NIH-3T3 Verified Samples in FCM: HepG2 Verified Samples in IP: Jurkat cells extracts |
| Dilution | WB 1:500-1:2000, IF 1:50, FCM 1:50-1:100, IP 1:25 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IF, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human HMG-CoA Reductase fragment |
| Abbre | HMGCR |
| Synonyms | LDLCQ, HMGCR, LDLCQ3, 3-hydroxy-3-methylglutaryl-CoA reductase, 3-hydroxy-3-methylglutaryl-CoA reductase, LDLCQ3, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase |
| Swissprot | |
| Calculated MW | 97 kDa |
| Observed MW |
97 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum, Peroxisome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Signal Transduction, Cancer, Metabolism |
| Clone No. | A254 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) is a transmembrane glycoprotein of the endoplasmic reticulum. It is the rate-limiting enzyme for cholesterol biosynthesis and non-sterol isoprenoid biosynthesis. Play a key role in cellular cholesterol homeostasis. The activity of HMGCR is regulated by negative feedback, in which cholesterol and other end products of the metabolic pathway inhibit the activity of HMGCR in a multivalent manner. Cholesterol inhibits reductase activity mainly by inhibiting the transcription rate of HMGCR gene. HMGCR is the main target of statins, which are a class of cholesterol-lowering drugs. |
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Unconjugated
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