Recombinant HNF-1B Monoclonal Antibody (AN301726L)
For research use only.
| Verified Samples | Verified Samples in WB: Jurkat, Mouse liver, Rat liver |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human HNF-1B fragment |
| Abbre | HNF-1B |
| Synonyms | LFB, HNF, HPC, LF-B, MODY, VHNF, TCF, HNF1B, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, TCF-2, TCF2, VHNF1, Hepatocyte nuclear factor 1 beta, Hepatocyte nuclear factor 1-beta, HNF 1B, HNF 2, HNF1 beta, HNF1 homeobox B, Homeoprotein LF B3, Homeoprotein LFB3, LF B3, MODY 5, TCF 2, TCF 2 protein, TCF2 protein, Transcription factor 2, Transcription factor 2 hepatic, Variant hepatic nuclear factor, Variant hepatic nuclear factor 1, VHNF 1 |
| Swissprot | |
| Calculated MW | 61 kDa |
| Observed MW |
61 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling, Metabolism |
| Clone No. | A434 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Hepatocyte nuclear factor 1-beta (HNF-1B) is a transcription factor which binds to specific regions of nucleic acid. HNF-1B, which plays critical roles in the regulation of glucose transport and metabolism, glucose-stimulated insulin secretion, and also in the embryonic development and later function of the pancreas, kidneys, genitourinary tract, liver, and the biliary tree. |
Other Clones
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Unconjugated
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