Recombinant Hsp60 Monoclonal Antibody (AN301009L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human liver tissue, Rat liver tissue, Rat mammary gland |
| Dilution | IHC 1:1000-1:4000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Hsp60 protein |
| Abbre | Hsp60 |
| Synonyms | CPN, HLD, SPG, Chaperonin, HSPD, HuCHA, GROEL, HLD4, HSP-60, HSP65, HuCHA60, SPG13, CPN60, Mitochond, 60 kDa chaperonin, Chaperonin 60, HSP60, HSPD1, 60 kDa heat shock protein, 60-KD, CH60, fa04a05, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat shock protein 60, Heat shock protein 65, heat shock protein family D (Hsp60) member 1, Hsp 60, HSP 65, mitochondrial, Mitochondrial matrix protein P1, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1 |
| Swissprot | |
| Calculated MW | 60 kDa |
| Observed MW |
60 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion matrix |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Kits, Lysates, Other, Isotype, Loading Controls |
| Clone No. | 1A7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. |
Other Clones
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Unconjugated
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