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For research use only.

Verified Samples Verified Samples in WB: K562, Rat Rat Brain, C6, 3T3
Verified Samples in IF: Human tonsil, Hela
Dilution WB 1:1000-1:2000,  IHC 1:50-1:100,  IF 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC-P,  IF
Clonality Rabbit Monoclonal
Immunogen A synthetic peptide of human Hsp70
Abbre Hsp70 1A
Synonyms HSP70-1B,  HSP70-2,  HSP70.2,  heat shock protein family A (Hsp70) member 1A,  heat shock protein family A (Hsp70) member 1B
Swissprot
Calculated MW 70 kDa
Observed MW 70 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoskeleton, centriole, centrosome, Cytosol, Endoplasmic reticulum, endoplasmic reticulum, Extracellular region or secreted, blood microparticle, extracellular exosome, extracellular region, Mitochondrion, Nucleus, nuclear speck, nucleoplasm, nucleus, Other locations: aggresome, cytoplasm, ficolin-1-rich granule lumen, focal adhesion, inclusion body, intracellular ribonucleoprotein complex, perinuclear region of cytoplasm, protein complex, vesicle.
Concentration 300 μg/mL
Buffer 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Purification Method Affinity Purified
Research Areas Cancer,  Signal Transduction
Clone No. R04-7A2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Other Clones

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Unconjugated

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