Recombinant IGF2R/CI-M6PR Monoclonal Antibody (AN301859L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Jurkat, C6, NIH/3T3 Verified Samples in IHC: Human thyroid cancer |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:200 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human IGF2R/CI-M6PR fragment |
| Abbre | IGF2R/CI-M6PR |
| Synonyms | MPR, IGF2R, CD222, CI-M6PR, CIMPR, M6P-R, M6P/IGF2R, MPR 300, MPR1, MPR300, MPRI, cation-independent mannose-6-phosphate receptor, IGF-IIR, 300 kDa mannose 6 phosphate receptor, 300 kDa mannose 6-phosphate receptor, cation independent, Cation independent mannose 6 phosphate receptor, CD222 antigen, CI Man 6 P receptor, CI Man-6-P receptor, CI MPR, CI-MPR, IGF 2 receptor, IGF 2R, IGF II receptor, IGF2 receptor, IGF-II receptor, Insulin like growth factor 2 receptor, Insulin like growth factor II receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P R, M6P/IGF2 receptor, M6PR, mannose 6 phosphate receptor |
| Swissprot | |
| Calculated MW | 274 kDa |
| Observed MW |
300 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Golgi apparatus membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Tags & Cell Markers, Signal Transduction |
| Clone No. | A571 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The HAP1 is a nuclear enzyme that apparently performs two distinct roles in the cellular defense against oxidative stress. One well-established role is in the repair of a variety of lesions induced in DNA either by spontaneous hydrolysis or by reactive oxygen species (ROS). This function has been characterized in great detail and the roles played by individual active site amino acid residues have been defined. The second role, which was identified only relatively recently and is still not characterized in detail, is to regulate the DNA binding activity of a group of nuclear factors. |
Other Clones
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