Recombinant IMPDH2 Monoclonal Antibody (AN301565L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, MCF-7, 4T1, HepG2 Verified Samples in IF: NIH/3T3 Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:1000, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human IMPDH2 fragment |
| Abbre | IMPDH2 |
| Synonyms | IMPD, IMPDH, IMPDH2, IMPD2, IMPDH-II, IMDH2, IMP (inosine monophosphate) dehydrogenase 2, IMP dehydrogenase 2, IMP oxireductase 2, IMPD 2, IMPDH 2, IMPDH II, Inosine 5' monophosphate dehydrogenase 2, Inosine 5' phosphate dehydrogenase 2, Inosine monophosphate dehydrogenase type II, Inosine-5''-monophosphate dehydrogenase 2 |
| Swissprot | |
| Calculated MW | 56 kDa |
| Observed MW |
56 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | A264 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Inosine-5'-monophosphate dehydrogenase 2, also known as IMP dehydrogenase 2, is an enzyme that in humans is encoded by the IMPDH2 gene.IMP dehydrogenase 2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. IMPDH2 catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 has been identified as an intracellular target of the natural product sanglifehrin A. |
Other Clones
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Unconjugated
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