Recombinant KIF5B Monoclonal Antibody (AN301889L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HepG2, Mouse brain, Rat brain Verified Samples in IHC: Human breast cancer |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human KIF5B fragment |
| Abbre | KIF5B |
| Synonyms | HEL-S, KIF5B, HEL-S-61, KINH, KNS, KNS1, UKHC |
| Swissprot | |
| Calculated MW | 110 kDa |
| Observed MW |
110 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton, Lysosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction |
| Clone No. | A605 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | KIF5B is a member of kinesin superfamily protein 5 (KIF5). KIF5 consists of three family members referred to as KIF5A, KIF5B, and KIF5C. KIF5A and KIF5C are specifically expressed in neurons whereas KIF5B is expressed ubiquitously. Targeted disruption of the kif5B gene resulted in embryonic lethality. These and other studies demonstrate that KIF5B plays an important role in the localization and distribution of major organelles, including the mitochondria and lysosome, and can contribute to autophagy. In addition, gene rearrangements involving KIF5B fusions to ALK and RET have been identified as drivers for lung cancer and other malignancies. |
Other Clones
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Other Formats
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Unconjugated
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