Recombinant LILRB1 Monoclonal Antibody (AN301773L)
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For research use only.
| Verified Samples |
Verified Samples in WB: THP-1, Ramos, A431, 小鼠脑, 大鼠脑 Verified Samples in IHC: Human liver Verified Samples in IF: PBMC, THP-1 Verified Samples in FCM: PBMC, Ramos |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:500, IF 1:50, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human LILRB1 fragment |
| Abbre | LILRB1 |
| Synonyms | LILRB, ILT, LIR, MIR, LILRB1, CD85J, ILT-2, ILT2, LIR-1, LIR1, MIR-7, MIR7, PIR-B, PIRB, ILT2, LILRB1, CD85, CD85 antigen, CD85 antigen like family member J, CD85 antigen-like family member J, CD85j antigen, Ig like transcript 2, ILT 2, immunoglobulin like transcript 2, Immunoglobulin-like transcript 2, leukocyte Ig like receptor 1, leukocyte immunoglobulin like receptor, Leukocyte immunoglobulin like receptor 1, leukocyte immunoglobulin like receptor subfamily B member 1, Leukocyte immunoglobulin like receptor subfamily B member 1 precursor, leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform, leukocyte immunoglobulin-like receptor, Leukocyte immunoglobulin-like receptor 1, Leukocyte immunoglobulin-like receptor subfamily B member 1, leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform, LIR 1, LIRB1, member 1, MIR 7, Monocyte/macrophage immunoglobulin like receptor 7, Monocyte/macrophage immunoglobulin-like receptor 7, PIR B, subfamily B, subfamily B (with TM and ITIM domains) |
| Swissprot | |
| Calculated MW | 71 kDa |
| Observed MW |
115, 19 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A481 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | LILRB1 is a member of the leukocyte immunoglobulin-like receptor (LIR) family. This protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. |
Other Clones
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