Recombinant Ly-6K Monoclonal Antibody (AN301716L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in FCM: MCF-7 |
| Dilution | WB 1:500-1:2000, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Ly-6K fragment |
| Abbre | Ly-6K |
| Synonyms | CO16, LY6K |
| Swissprot | |
| Calculated MW | 19 kDa |
| Observed MW |
19 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Cytoplasm and Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A424 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | LY6K is a member of LY6/urokinase-type plasminogen activator receptor family of proteins and has a glycosylphosphatidylinositol (GPI) anchor that results in its localization on the plasma membrane. LY6 gene perform a variety of functions in spermatogenesis, neutrophil activation, complement-mediated lysis, and macrophage biology. Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida. May play a role in cell growth. |
Other Clones
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