Recombinant LysRS Monoclonal Antibody (AN301592L)

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For research use only.
Verified Samples |
Verified Samples in WB: HeLa, JurKat, F9, C6 Verified Samples in IHC: Human testis, Mouse brain, Rat testis Verified Samples in IF: U-2 OS Verified Samples in IP: HeLa cells extracts |
Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50, IP 1:25-1:50 |
Isotype | IgG, κ |
Host | Rabbit |
Reactivity | Human, Rat, Mouse |
Applications | WB, IHC, IF, IP |
Clonality | Monoclonal;Recombinant |
Immunogen | Recombinant human LysRS fragment |
Abbre | LysRS |
Synonyms | DFNB, KIAA, KARS, CMTRIB, DFNB89, KARS1, KARS2, KRS, EC 6.1.1.6, KARS 1, KARS 2, Lysine tRNA ligase, Lysine--tRNA ligase, LysRS, Lysyl-tRNA synthetase, SYK, KIAA0070 |
Swissprot | |
Calculated MW | 75 kDa |
Observed MW |
75 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cell membrane, Cytoplasm, Mitochondrion, Nucleus, Secreted |
Concentration | 1 mg/mL |
Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
Purification Method | Protein A purified |
Research Areas | Epigenetics and Nuclear Signaling |
Clone No. | A291 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA3(Lys), the primer for reverse transcription initiation. |
Other Clones
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Other Formats
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Unconjugated
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