Recombinant MAGEA1 Monoclonal Antibody (AN301594L)
For research use only.
| Verified Samples |
Verified Samples in WB: A431 Verified Samples in IHC: Human testis Verified Samples in IF: HeLa |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human MAGEA1 fragment |
| Abbre | MAGEA1 |
| Synonyms | MAGE, MAGEA, MAGE1, MAGE1A, MAGEA1, Antigen MZ2 E, Antigen MZ2-E, Cancer/testis antigen 1.1, cancer/testis antigen family 1, CT1.1, MAGA1, MAGE 1 antigen, MAGE-1 antigen, Melanoma antigen 1, Melanoma antigen family A 1, Melanoma antigen family A 1 (directs expression of antigen MZ2 E), Melanoma antigen MAGE 1, Melanoma associated antigen 1, Melanoma associated antigen MZ2 E, Melanoma-associated antigen 1, member 1, MGC9326 |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
46 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Cancer |
| Clone No. | A293 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. |
Other Clones
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Other Formats
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Unconjugated
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