Recombinant MCM7 Monoclonal Antibody (AN302062L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa,?Jurkat Verified Samples in IF: MCF-7, HeLa Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:20000-1:50000, IF 1:100, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | MCM7 |
| Synonyms | CDC, PNAS, P1CDC, PPP1R, P1.1-MCM, MCM, MCM7, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, formerly, homolog of, regulatory subunit 104, S. cerevisiae, CDABP0042, CDC 47, CDC47 homolog, DNA replication licensing factor MCM7, Homolog of S. cerevisiae Cdc47, MCM 2, MCM 7, MCM7 minichromosome maintenance deficient 7, MCM7_HUMAN, Minichromosome maintainence, Minichromosome Maintainence 7, Minichromosome maintenance complex component 7, Minichromosome maintenance deficient 7, Minichromosome maintenance protein 7, P1.1 MCM3, PNAS 146, Protein phosphatase 1 |
| Swissprot | |
| Calculated MW | 81 kDa |
| Observed MW |
81 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A782 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
Other Clones
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Unconjugated
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