Recombinant MEF2C Monoclonal Antibody (AN302041L)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat (negative?control),?K562, Ramos Verified Samples in IHC: Mouse spleen, Rat spleen |
| Dilution | WB 1:5000, IHC 1:500 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | MEF2C |
| Synonyms | DEL5q, C5DELq, MEF2C, C5DELq14.3, DEL5q14.3, Myocyte-specific enhancer factor 2C, MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C), Myocyte enhancer factor 2C, Myocyte specific enhancer factor 2C, OTTHUMP00000222409, Similar to MADS box transcription enhancer factor 2 polypeptide C |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Neuroscience, Epigenetics and Nuclear Signaling, Stem Cells, Cardiovascular |
| Clone No. | A761 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | MEF2C is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, there are four MEF2C-related genes (MEF2A, MEF2B, MEF2C and MEF2D) that encode proteins that exhibit significant amino acid sequence similarity within their DNA binding domains and, to a lesser extent, throughout the rest of the proteins. The MEF2 family members were originally described as muscle specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes. Recently, several groups have reported MEF2 binding activity and MEF2 proteins in a wide variety of cell types where these proteins appear to play an important role in growth factor- and stress-induced early gene responses. |
Other Clones
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Unconjugated
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