Recombinant MICB Monoclonal Antibody (AN300058P)
For research use only.
| Dilution | WB: 1:1000；FC: 1:50；IP: 1:20-1:50 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, FC, IP |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human MICB protein |
| Abbre | MICB |
| Synonyms | PERB, MICB, PERB11.2, MHC class I polypeptide-related sequence B |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW |
50-75 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology |
| Clone | A1168 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | MICA and MICB proteins are stress - inducible molecules. They are expressed on the surface of cells under various stress conditions, such as infection, inflammation, or cellular transformation. Their main function is to serve as ligands for the NKG2D receptor, which is expressed on natural killer (NK) cells, cytotoxic T lymphocytes (CTLs), and some other immune cells. When MICA or MICB binds to NKG2D, it triggers an activating signal in the immune cells, leading to the activation of their cytotoxic functions. This helps the immune system to identify and eliminate stressed, infected, or transformed cells. |
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