Recombinant MMP2 Monoclonal Antibody (AN300712L)
For research use only.
| Verified Samples | Verified Samples in WB: U-87 MG |
| Dilution | WB 1:2000-1:10000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human MMP2 protein |
| Abbre | MMP2 |
| Synonyms | TBE, CLG, Matrix Metalloproteinase, MMP, CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1, MMP2, 72 kDa Gelatinase, 72 kDa Type IV Collagenase, Gelatinase A, Matrix Metalloproteinase-2, 72kD type IV collagenase, 72kDa gelatinase, 72kDa type IV collagenase), CLG 4, CLG 4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase, Matrix Metalloproteinase 2, Matrix metalloproteinase 2 (gelatinase A, Matrix metalloproteinase II, MMP 2, MMP II, Neutrophil gelatinase, PEX, TBE 1, MMP-2 |
| Swissprot | |
| Calculated MW | 74 kDa |
| Observed MW |
64 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Specificity | Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Cancer, Cell Biology, Metabolism |
| Clone No. | 12G7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Matrix metallopeptidase 2(MMP2) Homo sapiens This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. |
Other Clones
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Unconjugated
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