Recombinant mtTFA Monoclonal Antibody (E-AB-81584)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Hela Verified Samples in IF: Human breast cancer, Hela |
| Dilution | WB 1:1000-1:2000, IHC 1:50-1:100, IF 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human mtTFA |
| Abbre | mtTFA |
| Synonyms | Mitochondrial transcription factor 1, MtTF1, TCF 6, TCF-6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Tra, Transcription factor 6, Transcription factor 6 like 2 (mitochondrial transcription factor), anscription factor 6-like 1, mitochondrial transcription factor A, mtTFA |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
24 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction, Tags and Cell Markers |
| Clone No. | R08-3E2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. TFAM (Transcription Factor A, Mitochondrial) is a Protein Coding gene. Diseases associated with TFAM include Mitochondrial Dna Depletion Syndrome 15, Hepatocerebral Type and Skin Carcinoma In Situ. Among its related pathways are Mitochondrial Gene Expression and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins. GO annotations related to this gene include poly(A) RNA binding and chromatin binding. |
Other Clones
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Other Formats
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Unconjugated
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