Recombinant Na+/K+ ATPase alpha-1 Monoclonal Antibody (AN301712L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, RAW264.7, C6, Mouse brain, Rat brain Verified Samples in IHC: Human cervical cancer, Human kidney Verified Samples in IF: Jurkat, LNCaP |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Na+/K+ ATPase alpha-1 fragment |
| Abbre | Na+/K+ ATPase alpha-1 |
| Synonyms | ATPase Na+/K+ transporting subunit alpha, HOMGSMR, ATP1A1, ATPase Na+/K+ transporting subunit alpha 1, ATP1A, CMT2DD, HOMGSMR2, A1A1, AT1A1, Atpa-1, ATPase Na+/K+ transporting alpha 1 polypeptide, BC010319, EC 3.6.3.9, K ATPase alpha 1 subunit, K+ ATPase alpha subunit, MGC3285, MGC38419, MGC51750, Na K ATPase alpha A catalytic polypeptide, Na K ATPase catalytic subunit alpha A protein, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na+, Na+/K+ ATPase alpha 1 subunit, Na+/K+ ATPase 1, Nkaa1b, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump 1, Sodium pump subunit alpha-1, Sodium/potassium-transporting ATPase subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-1 |
| Swissprot | |
| Calculated MW | 113 kDa |
| Observed MW |
113 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane and Melanosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Signal Transduction, Neuroscience, Cancer, Metabolism, Isotype, Loading Controls |
| Clone No. | A420 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. |
Other Clones
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Unconjugated
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