Recombinant OLR1/LOX1 Monoclonal Antibody (AN301759L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human placenta Verified Samples in IF: TF-1 Verified Samples in FCM: HUVEC |
| Dilution | WB 1:1000-1:5000, IF 1:50, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human OLR1/LOX1 fragment |
| Abbre | OLR1/LOX1 |
| Synonyms | OLR, Ox-LDL receptor, lectin-type oxidized LDL receptor, Oxidized low-density lipoprotein receptor, SLOX, SCARE, lectin-type oxidized LDL receptor 1, LOX-1, LOXIN, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, SCARE1, SLOX1, CLEC8A, LOX1, OLR1, C-type lectin domain family 8 member A, hLOX 1, hLOX-1, Lectin like oxidized LDL receptor 1, Lectin like oxLDL receptor 1, Lectin type oxidized LDL receptor 1, Lectin-like oxidized LDL receptor 1, Lectin-like oxLDL receptor 1, low density lipoprotein oxidized, member 1, Ox LDL receptor 1, Oxidised low density lipoprotein (lectin like) receptor 1, Oxidized low density lipoprotein receptor 1, Oxidized low density lipoprotein receptor 1 soluble form, receptor 1, Scavenger receptor class E, soluble form, SR-EI |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane and Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Metabolism |
| Clone No. | A467 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This protein is a low density lipoprotein receptor that belongs to the C-type lectin superfamily. It is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants. Diseases associated with OLR1 include Myocardial Infarction and Chlamydia Pneumonia. Among its related pathways are Innate Immune System and Response to elevated platelet cytosolic Ca2+. Gene Ontology (GO) annotations related to this gene include carbohydrate binding and low-density lipoprotein particle receptor activity. |
Other Clones
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Unconjugated
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