Recombinant p21 Monoclonal Antibody (AN301027L)
For research use only.
| Verified Samples |
Verified Samples in WB: HCT-116 Verified Samples in IHC: Human cervical carcinoma tissue |
| Dilution | IHC 1:100-1:200, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human p21 protein |
| Abbre | P21 |
| Synonyms | WAF, SDI, CIP, CAP, PIC, CDKN, CDK-interacting protein, cyclin-dependent kinase inhibitor, Melanoma differentiation-associated protein, p21CIP, Slc12a, MDA, CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, cyclin-dependent kinase inhibitor 1, CDKI, CDK-interacting protein 1, CDN1A, Cyclin Dependent Kinase Inhibitor 1A, DNA Synthesis Inhibitor, Melanoma differentiation-associated protein 6, P21waf, SLC12A9, FASN, FAS, MDA6, PIC1, CAP20, CDKN1, CIP1, cyclin-dependent kinase inhibitor 1, MDA-6, P21, p21CIP1, SDI1, WAF1, cyclin-dependent kinase inhibitor 1A |
| Swissprot | |
| Calculated MW | 18 kDa |
| Observed MW |
18 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Cancer, Kits, Lysates, Other |
| Clone No. | 5A2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. |
Other Clones
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Unconjugated
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