Recombinant p60 CAF1/MPP7 Monoclonal Antibody (AN301745L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, K562 Verified Samples in IHC: Human tonsil Verified Samples in IF: 293T, TF-1 Verified Samples in IP: Jurkat cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human p60 CAF1 / MPP7 fragment |
| Abbre | p60 CAF1/MPP7 |
| Synonyms | MPP, CAF1P, CAF-IP, MPHOSPH, CAF, CHAF1B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7 |
| Swissprot | |
| Calculated MW | 61 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A453 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Chromatin assembly factor 1 p60 (p60 CAF1) is a subunit of Chromatin assembly factor I (CAF-I) which is required for the assembly of histone octamers onto newly-replicated DNA. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. |
Other Clones
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Other Formats
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Unconjugated
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