Recombinant PAKγ Monoclonal Antibody (AN301192L)
For research use only.
| Verified Samples |
Verified Samples in WB: K562 Verified Samples in IHC: Human breast carcinoma |
| Dilution | IHC 1:200-1:1000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human PAKγ protein |
| Abbre | PAKγ |
| Synonyms | PAK, PAK2, PAK65, PAKgamma, 65-KD, CB422, C-t-PAK2, EC 2.7.11.1, Gamma PAK, Gamma-PAK, hPAK65, Kinase, p21 (CDKN1A) activated kinase 2, p21 (CDKN1A)-activated kinase 2a, p21 activated kinase 2, p21 protein (Cdc42/Rac)-activated kinase 2, p21 protein Cdc42 Rac activated kinase 2, p21-activated kinase, p21-activated kinase 2, p21-activated protein kinase I, p21CDKN1A activated kinase 2, p27, p34, p58, p65PAK, PAK 2, PAK-2, PAK-2p34, S6 H4 kinase, S6/H4 kinase, Serine threonine protein kinase PAK 2, Serine/threonine protein kinase PAK 2, PAK2 |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW |
58 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane, Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Microbiology, Signal Transduction, Cancer, Neuroscience |
| Clone No. | 5D5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. |
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