Recombinant Phospho-ATM (Ser1981) Monoclonal Antibody (AN301139L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela treated with UV for 1 hour Verified Samples in IHC: Human breast carcinoma |
| Dilution | IHC 1:200-1:1000, WB 1:10000-1:50000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide corresponding to residues around (Ser1981) of Human Phospho-ATM |
| Abbre | Atm |
| Synonyms | TEL, TELO, AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1, ATM, AT mutated, A-T mutated, A-T mutated homolog, Ataxia telangiectasia mutated, Ataxia telangiectasia mutated gene, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated homolog (human), ATM serine/threonine kinase, DKFZp781A0353, homolog, MGC74674, OTTHUMP00000232981, Serine protein kinase ATM, Serine/threonine-protein kinase ATM, Serine-protein kinase ATM, Tefu, Telomere fusion protein, telomere maintenance 1 |
| Swissprot | |
| Calculated MW | 351 kDa |
| Observed MW |
351 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasmic vesicle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 11A9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. |
Other Clones
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Unconjugated
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