Recombinant Phospho-c-Myc (Ser62) Monoclonal Antibody (AN302092L)
For research use only.
| Verified Samples | Verified Samples in WB: HCT-116 |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | phosphorylated human c-Myc (Ser62) peptide |
| Abbre | Phospho-c-Myc (Ser62) |
| Synonyms | bHLHe, MYC BHLHE, MRTL, MYCC, bHLHe39, c-Myc, MYC, MYC BHLHE39, AU016757, Avian myelocytomatosis viral oncogene homolog, c Myc, Class E basic helix-loop-helix protein 39, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, Myc2, myc-related translation/localization regulatory factor, Myelocytomatosis oncogene, Niard, Nird, Oncogene Myc, OTTHUMP00000158589, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian), V-myc myelocytomatosis viral oncogene homolog(avian) |
| Swissprot | |
| Calculated MW | 49 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling, Stem Cells, Cancer |
| Clone No. | A816 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Myc, also known as c-Myc, together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene. Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types. Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. Myc is also expressed during embryogenesis. Myc is upregulated in many cancer types, especially in aggressive, poorly differentiated tumors. Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma. In addition, Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation. |
Other Clones
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