Recombinant Phospho-FOXO3A (Ser253) Monoclonal Antibody (AN301011L)
For research use only.
| Verified Samples |
Verified Samples in WB: HEK293 Verified Samples in IHC: Human pancreas tissue, Mouse pancreas tissue |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide corresponding to residues around (Ser253) of Human Phospho-FOXO3A |
| Abbre | FoxO3A |
| Synonyms | AF6q, FKHRL, FKHRL1P, FOXO3, AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, AF6q21 protein, DKFZp781A0677, FKHR2, FKHRL 1, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, Forkhead box O3, Forkhead box O3A, Forkhead box protein O3, Forkhead box protein O3A, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead homolog (rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead in rhabdomyosarcoma-like 1, FOX O3A, MGC12739, MGC31925 |
| Swissprot | |
| Calculated MW | 71 kDa |
| Observed MW |
97 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Cell Biology, Cancer, Metabolism |
| Clone No. | 1A9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. |
Other Clones
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Unconjugated
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