Recombinant Phospho-p90RSK (Thr359, Ser363) Monoclonal Antibody (AN300156L)
For research use only.
| Verified Samples | Verified Samples in WB: A431 |
| Dilution | WB 1:10000-1:100000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide corresponding to the residues around (Thr359, Ser363) of Human p90RSK |
| Abbre | RPS6KA1 |
| Synonyms | RPS6KA, HU-1, MAPKAPK1A, RSK, RSK1, p90Rsk, RPS6KA1, 90 kDa ribosomal protein S6 kinase 1, 90kD, dJ590P13.1, dJ590P13.1 (ribosomal protein S6 kinase, EC 2.7.11.1, HU 1, HU1, KS6A1, MAP kinase activated protein kinase 1a, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, MGC79981, Mitogen-activated protein kinase-activated protein kinase 1A, OTTHUMP00000004113, p90 RSK1, p90-RSK 1, p90RSK1, p90S6K, polypeptide 1, pp90RSK1, Ribosomal protein S6 kinase 90kD 1, Ribosomal protein S6 kinase 90kD polypeptide 1, Ribosomal protein S6 kinase 90kDa polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase alpha-1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal S6 kinase 1, RPS6K1 alpha, RSK 1, RSK 1 p90, RSK-1, S6K alpha 1, S6K-alpha-1 |
| Swissprot | |
| Calculated MW | 83 kDa |
| Observed MW |
83 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5 |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling |
| Clone No. | 5B8 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 1 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
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