Recombinant PPP1CB Monoclonal Antibody (AN301634L)
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For research use only.
| Verified Samples |
Verified Samples in WB: A431, Jurkat, NIH/3T3, PC-12, Mouse brain, Rat brain Verified Samples in IHC: Human cervical cancer Verified Samples in IF: HeLa, NIH/3T3 Verified Samples in FCM: HeLa Verified Samples in IP: Jurkat cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:200-1:500, FCM 1:50-1:100, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PPP1CB fragment |
| Abbre | PPP1CB |
| Synonyms | NSLH, PPP1CB, HEL-S-80p, PP-1B, PP1B, PP1beta, PPP1CD, NSLH2, beta isozyme, catalytic subunit, delta isoform, MGC3672, PP 1B, Protein phosphatase 1, Protein phosphatase 1 beta, Protein phosphatase 1 catalytic subunit beta isoform, Protein phosphatase 1 delta, Serine threonine protein phosphatase PP1 beta catalytic subunit, Serine/threonine-protein phosphatase PP1-beta catalytic subunit |
| Swissprot | |
| Calculated MW | 37 kDa |
| Observed MW |
35 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism, Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | A337 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective. |
Other Clones
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Unconjugated
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