Recombinant PRAME Monoclonal Antibody (AN301801L)
For research use only.
| Verified Samples | Verified Samples in WB: Ramos, K562, HeLa, A375 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PRAME fragment |
| Abbre | PRAME |
| Synonyms | OIP, PRAME, CT130, MAPE, OIP-4, OIP4, 4930534P07Rik, Cancer/testis antigen 130, Melanoma antigen preferentially expressed in tumors, OIP 4, OIP4, OPA interacting protein 4, Opa interacting protein OIP4, OPA-interacting protein 4, Preferentially expressed antigen in melanoma, Preferentially expressed antigen of melanoma, RP23-250F8.3 |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW |
58 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Cytoplasm, Golgi apparatus, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | A513 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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