Recombinant PRMT5 Monoclonal Antibody (AN301635L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HEK-293, MCF7, HepG2 Verified Samples in IHC: Human breast cancer, Human liver cancer Verified Samples in IP: MCF7 cells extracts |
| Dilution | WB 1:2000-1:10000, IHC 1:50-1:100, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PRMT5 fragment |
| Abbre | PRMT5 |
| Synonyms | IBP, JBP, SKB, PRMT, HRMT1L, HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, PRMT5, 72 kDa ICln binding protein, 72 kDa ICln-binding protein, ANM5, Histone synthetic lethal 7, Histone-arginine N-methyltransferase PRMT5, HMT1 hnRNP methyltransferase like 5, homolog of, Jak-binding protein 1, JBP 1, PRMT 5, Protein arginine methyltransferase 5, Protein arginine N methyltransferase 5, Protein arginine N methyltransferase 5 N terminally processed, Protein arginine N-methyltransferase 5, S. cerevisiae, S. POMBE, S. POMBE HOMOLOG OF, SHK1 KINASE BINDING PROTEIN 1, Shk1 kinase binding protein 1 homolog, Shk1 kinase/binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB 1, SKB1 homolog, SKB1: SKB1 homolog (S. pombe) |
| Swissprot | |
| Calculated MW | 73 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Chromosome, Cytoplasm, Golgi apparatus, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A338 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. |
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Unconjugated
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