Recombinant Prothrombin Monoclonal Antibody (AN301787L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human liver, Human placenta, Human plasma Verified Samples in IHC: Human liver |
| Dilution | WB 1:1000-1:5000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Prothrombin fragment |
| Abbre | Prothrombin |
| Synonyms | THPH, RPRGL, F2, PT, RPRGL2, THPH1, coagulation factor II, F1+2, PIVKA-Ⅱ, Prothrombin, Abnormal prothrombin, APT, DCP, Des-γ-Carboxy Prothrombin, protein induced by vitamin K absence or antagonist-Ⅱ |
| Swissprot | |
| Calculated MW | 70 kDa |
| Observed MW |
72, 52, 33 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Cardiovascular, Signal Transduction |
| Clone No. | A495 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Prothrombin is a protein made by the liver. It is a coagulation (clotting) factor that is needed for the normal clotting of blood. A cascade of biochemical events leads to the formation of the final clot. In this cascade, prothrombin is a precursor to thrombin. Prothrombin time (PT) is a blood test that measures how long it takes blood to clot. A prothrombin time test can be used to check for bleeding problems. PT is also used to check whether medicine to prevent blood clots is working. |
Other Clones
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Unconjugated
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