Recombinant RAP1A+RAP1B Monoclonal Antibody (AN301751L)
For research use only.
| Verified Samples |
Verified Samples in WB: HEK-293, C6, NIH/3T3, Mouse brain, Rat brain Verified Samples in IF: HeLa Verified Samples in IP: HEK-293 cells extracts |
| Dilution | WB 1:1000-1:2000, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human RAP1A+RAP1B fragment |
| Abbre | RAP1A+RAP1B |
| Synonyms | RAP, SMGP, Ras-related protein Krev, RAP1A, C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21, GTP-binding protein smg p21A, member of RAS oncogene family, Ras-related protein 1A, Ras-related protein Krev-1, Ras-related protein Rap-1A |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
21 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Kits, Lysates, Other, Epigenetics and Nuclear Signaling, Tags & Cell Markers |
| Clone No. | A459 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Rap1 is a member of the Ras family of small GTPases. It undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of Rap1 is therefore affected by the balance of intracellular levels of GEFs and GAPs. This protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. |
Other Clones
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Other Formats
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Unconjugated
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