Recombinant RUNX2 Monoclonal Antibody (AN300866L)
For research use only.
| Verified Samples | Verified Samples in WB: MDA-MB-231 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human RUNX2 protein |
| Abbre | RUNX2 |
| Synonyms | AML, RUNX, CBFA, CBF-alpha, OSF, RUNX2, AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA, PEBP2A1, PEBP2A2, PEBP2aA1, PEBP2A, Acute myeloid leukemia 3 protein, Alpha subunit 1, CBF alpha 1, Cleidocranial dysplasia 1, Core binding factor, Core binding factor runt domain alpha subunit 1, Core binding factor subunit alpha 1, Core-binding factor subunit alpha-1, MGC120022, MGC120023, Oncogene AML 3, Oncogene AML-3, OSF 2, Osteoblast specific transcription factor 2, Osteoblast-specific transcription factor 2, OTTHUMP00000016533, PEA2 alpha A, PEA2-alpha A, PEBP2 alpha A, PEBP2-alpha A, Polyomavirus enhancer binding protein 2 alpha A subunit, Polyomavirus enhancer-binding protein 2 alpha A subunit, Runt domain, Runt related transcription factor 2, Runt-related transcription factor 2, SL3 3 enhancer factor 1 alpha A subunit, SL3/AKV core binding factor alpha A subunit, SL3/AKV core-binding factor alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit |
| Swissprot | |
| Calculated MW | 57 kDa |
| Observed MW |
57 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology, Neuroscience, Cancer |
| Clone No. | 8B9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. |
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