Recombinant S100A1 Monoclonal Antibody (AN301707L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse heart Verified Samples in IHC: Human cerebrum, Human melanoma Verified Samples in IF: C2C12 Verified Samples in FCM: C2C12 |
| Dilution | WB 1:500, IHC 1:200-1:1000, IF 1:50, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Synthetic peptide derived from human S100A1 protein |
| Abbre | S100A1 |
| Synonyms | S100A, S100A1, Bpb, NEF, Protein S100-A1, S100, S100 alpha, S100 beta, S100 calcium binding protein A1, S100 calcium binding protein B, S100 calcium-binding protein A1, S-100 protein alpha chain, S100 protein alpha polypeptide, S-100 protein subunit alpha, S100-alpha, S100B, S100beta, S10A1 |
| Swissprot | |
| Calculated MW | 11 kDa |
| Observed MW |
11 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm and nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction, Tags & Cell Markers |
| Clone No. | A415 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | S100A1 is a member of the S100 protein family, each of which contains two EF-hand calcium-binding motifs. These proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. At least 13 S100 genes are clustered on chromosome 1q21. S100A1 may function in stimulation of Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. |
Other Clones
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Unconjugated
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