Recombinant S100A8 Monoclonal Antibody (AN301719L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Rat spleen Verified Samples in IHC: Human tonsil, Mouse spleen, Rat spleen Verified Samples in FCM: THP-1 |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:1000, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human S100A8 fragment |
| Abbre | S100A8 |
| Synonyms | Protein S100-A, S100a, Kcnip, KCHIP, MRP, S100A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, Calgranulin A, Calgranulin-A, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Protein S100-A8, CALP, KCNIP4, KCHIP4, AI323541, B8Ag, BEE11, Calprotectin, Calprotectin L1L subunit, Chemotactic cytokine CP-10, included, Migration inhibitory factor-related protein 8, Myeloid-related protein 8, Neutrophil cytosolic 7 kDa protein, Pro-inflammatory S100 cytokine, S100 calcium binding protein A8, S100 calcium binding protein A8 (calgranulin A), S100 calcium-binding protein A8, S100A8/S100A9 complex, S10A8, Urinary stone protein band A |
| Swissprot | |
| Calculated MW | 11 kDa |
| Observed MW |
11 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Cytoplasm, Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Cancer, Immunology, Signal Transduction, Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | A427 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | MRP8/S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. MRP8 has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. |
Other Clones
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