Recombinant S100A9 Monoclonal Antibody (AN301742L)
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For research use only.
| Verified Samples |
Verified Samples in WB: THP-1 Verified Samples in IHC: Human spleen, Human tonsil Verified Samples in IF: PBMC |
| Dilution | WB 1:500-1:2000, IHC 1:500-1:2000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human S100A9 fragment |
| Abbre | S100A9 |
| Synonyms | MAC, Protein S100-A, S100 calcium-binding protein A, Migration inhibitory factor-related protein, S100A, MRP, S100A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, Protein S100-A9, S100 calcium-binding protein A9, Calgranulin B, Cystic fibrosis antigen B, Migration inhibitory factor related protein 14, MRP 14, Myeloid-related protein 14, OTTHUMP00000015331, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S10A9 |
| Swissprot | |
| Calculated MW | 13 kDa |
| Observed MW |
13 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Cytoplasm, Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Signal Transduction, Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | A450 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. |
Other Clones
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