Recombinant SF3B3 Monoclonal Antibody (AN301841L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Jurkat, HEK-293, RAW 264.7, C6 Verified Samples in IHC: Mouse colon, Rat cerebrum Verified Samples in IP: Jurkat cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:500-1:2000, IP 1:25 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human SF3B3 fragment |
| Abbre | SF3B3 |
| Synonyms | SAP, SF3B, KIAA, Splicing factor 3B subunit, Spliceosome-associated protein, Spliceosome-associated protein 130, Splicing factor 3B subunit 3, SF3B3, KIAA0017, SAP130, Pre mRNA splicing factor SF3b 130 kDa subunit, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP 130, SF3b130, SF3B3_HUMAN, Spliceosome associated protein 130, Splicing factor 3b subunit 3 130kD, STAF130 |
| Swissprot | |
| Calculated MW | 135 kDa |
| Observed MW |
135 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A553 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | SF3B3 is one of four proteins for SF3B, which is involved in RNA splicing. Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occurred in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which composed of 4 proteins. SF3B3 is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence(BPS) in pre-mRNA. |
Other Clones
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