Recombinant SLC25A12 Monoclonal Antibody (AN301939L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Mouse skeletal muscle Verified Samples in IHC: Mouse cardiac muscle, Rat cardiac muscle |
| Dilution | WB 1:2000-1:5000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human SLC25A12 fragment |
| Abbre | SLC25A12 |
| Synonyms | AGC, EIEE, SLC25A, SLC25A12, AGC1, ARALAR, EIEE39, ARALAR1, Araceli hiperlarga, Calcium binding mitochondrial carrier superfamily member, Calcium binding mitochondrial carrier superfamily member Aralar1, Calcium-binding mitochondrial carrier protein Aralar1, CMC1, member 12, Mitochondrial aspartate glutamate carrier 1, solute carrier family 25, Solute carrier family 25 (aspartate/glutamate carrier) member 12, Solute carrier family 25 (mitochondrial carrier Aralar) member 12, Solute carrier family 25 member 12 |
| Swissprot | |
| Calculated MW | 75 kDa |
| Observed MW |
75 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Tags & Cell Markers, Signal Transduction, Metabolism |
| Clone No. | A655 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The calcium-binding mitochondrial carrier protein (SLC25A12), also named as ARALAR or AGC1, is an aspartate-glutamate exchange protein responsible for transporting mitochondrial aspartate across the mitochondrial inner membrane in exchange for cytosolic glutamate. SLC25A12 and other proteins of the aspartate-glutamate carrier (AGC) group are required for the transfer of mitochondrial aspartate to the cytosol, a key step in urea synthesis. Research studies using SLC25A12-knockout mice indicate that SLC25A12 plays an important role in proper CNS myelination. Mice lacking SLC25A12 suffer from hypomyelination as a result of a lack of oligodendrocyte maturation caused by decreased brain N-acetylaspartate levels. Mutation of the corresponding SLC25A12 gene can result in global cerebral hypomyelination and severe psychomotor retardation, caused by deficient SLC25A12 activity and limited mitochondrial aspartate efflux. |
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Unconjugated
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