Recombinant Smac/Diablo Monoclonal Antibody (AN301658L)

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For research use only.
Verified Samples |
Verified Samples in WB: Jurkat, MCF-7,HepG2, C2C12, C6 Verified Samples in IHC: Human lung squamous carcinoma Verified Samples in IF: HeLa Verified Samples in FCM: Jurkat Verified Samples in IP: Jurkat cells extracts |
Dilution | WB 1:500-1:2000, IHC 1:50-1:100, IF 1:500-1:1000, FCM 1:50-1:100, IP 1:25-1:100 |
Isotype | IgG, κ |
Host | Rabbit |
Reactivity | Human, Rat, Mouse |
Applications | WB, IHC, IF, FCM, IP |
Clonality | Monoclonal;Recombinant |
Immunogen | Recombinant human Smac/Diablo fragment |
Abbre | Smac/Diablo |
Synonyms | DFNA, DIABLO, DFNA64, SMAC, 0610041G12Rik, DBLOH, DBOH, Diablo homolog, Diablo homolog (Drosophila), Diablo homolog mitochondrial, Diablo IAP binding mitochondrial protein, Diablo like protein, DIABLO S, Direct IAP binding protein with low pI, Direct IAP-binding protein with low pI, FLJ10537, FLJ25049, mitochondrial, Mitochondrial Smac protein, Second mitochondria derived activator of caspase, Second mitochondria-derived activator of caspase, SMAC 3, Smac protein, SMAC3 |
Swissprot | |
Calculated MW | 27 kDa |
Observed MW |
21 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Mitochondrion |
Concentration | 1 mg/mL |
Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
Purification Method | Protein A purified |
Research Areas | Cell Biology, Cancer, Metabolism |
Clone No. | A361 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
Other Clones
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Unconjugated
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