Recombinant SOD2 (Acetyl Lys68) Monoclonal Antibody (AN301412L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human liver, Rat kidney Verified Samples in IHC: Human colon cancer, Mouse liver |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Acetylated human histone SOD2 (Lys68) peptide |
| Abbre | SOD2 (Acetyl Lys68) |
| Synonyms | SOD, MVCD, superoxide dismutase, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, SOD2, Superoxide Dismutase [Mn] Mitochondrial, superoxide dismutase 2, IPOB, IPO-B, MNSOD, Mn-SOD, MVCD6, superoxide dismutase 2, EC:1.15.1.1, mitochondrial, Superoxide dismutase [Mn] |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
20 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion matrix |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Signal Transduction, Neuroscience, Cardiovascular, Cancer, Metabolism |
| Clone No. | A107 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Manganese superoxide dismutase (MnSOD), also named as SOD2, is a mitochondrial detoxification enzyme that catalyzes the conversion of superoxide to hydrogen peroxide. Hydrogen peroxide is then decomposed to water by catalase, glutathione peroxidase, or peroxiredoxins. MnSOD and other enzymes involved in antioxidant defense protect cells from reactive oxygen species (ROS). Calorie restriction leads to SIRT3-mediated deacetylation of MnSOD and the subsequent increase of its antioxidant activity. MnSOD also plays an essential role in mediating the protective effect of mTOR inhibition to reduce epithelial stem cell senescence. |
Other Clones
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Unconjugated
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