Recombinant SQSTM1/p62 Monoclonal Antibody (AN301383L)
For research use only.
| Verified Samples | Verified Samples in WB: PANC-1 |
| Dilution | WB 1:500-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human SQSTM1/p62 protein |
| Abbre | SQSTM1/p62 |
| Synonyms | ZIP, OSF, EBI 3 associated protein p, STONE, FTDALS, PDB, SQSTM1, A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1, EBI 3 associated protein p60, EBIAP, OSF-6, PDB 3, Sequestosome 1, SQSTM 1, STAP, STONE14, ORCA, A170, DMRV, FTDALS3, NADGP, OSIL, p60, p62, p62B, PDB3, sequestosome-1, ZIP3, EBI 3 associated protein of 60 kDa, EBI3 associated protein of 60 kDa, EBI3 associated protein p60, EBI3-associated protein of 60 kDa, MGC127197, Osi, Oxidative stress induced like, Paget disease of bone 3, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa, Phosphotyrosine independent ligand for the Lck SH2 domain p62, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, PKC-zeta-interacting protein, Protein kinase C-zeta-interacting protein, SQSTM, Ubiquitin binding protein p62, Ubiquitin-binding protein p62, ZIP 3 |
| Swissprot | |
| Calculated MW | 48 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nuclear |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cardiovascular, Metabolism, Cancer |
| Clone No. | 10A5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. |
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Unconjugated
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