Recombinant Stathmin Monoclonal Antibody (AN301665L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, Neuro-2a, Mouse brain Verified Samples in IHC: Human lung squamous carcinoma, Human tonsil, Mouse cerebrum, Rat cerebrum Verified Samples in IF: Jurkat Verified Samples in IP: Jurkat cells extracts |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Stathmin fragment |
| Abbre | Stathmin |
| Synonyms | LAP, STMN, Protein Pr, Oncoprotein, Phosphoprotein p, Leukemia-Associated Phosphoprotein p, C1orf, STMN1, C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, stathmin, Leukemia-Associated Phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, STMN1, C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, stathmin |
| Swissprot | |
| Calculated MW | 17 kDa |
| Observed MW |
18 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction |
| Clone No. | A368 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. |
Other Clones
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Other Formats
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Unconjugated
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