Recombinant SUV39H2 Monoclonal Antibody (AN301817L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HEK-293T, MOLT-4, NIH/3T3 Verified Samples in IHC: Mouse testis, Rat testis |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human SUV39H2 fragment |
| Abbre | SUV39H2 |
| Synonyms | SUV39H, SUV39H2, KMT1B, FLJ23414, H3 K9 HMTase 2, H3-K9-HMTase 2, Histone H3 K9 methyltransferase 2, Histone H3-K9 methyltransferase 2, Histone lysine N methyltransferase H3 lysine 9 specific 2, Histone lysine N methyltransferase SUV39H2, Histone-lysine N-methyltransferase SUV39H2, Lysine N methyltransferase 1B, Lysine N-methyltransferase 1B, sSuppressor of variegation 3 9 homolog 2 (Drosophila), Su(var)3 9 Drosophila homolog of 2, Su(var)3 9 homolog 2, Su(var)3-9 homolog 2, Suppressor of variegation 3 9 homolog 2, Suppressor of variegation 3-9 homolog 2, SUV92 |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
47, 50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A529 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | SUV39H2, also known as KMT1B, is a member of the SUV39 subfamily of lysine methyltransferases (KMTs) that plays a significant role in histone H3-K9 di-/Trimethylation, transcriptional regulation and cell cycle. Overexpressions of SUV39H2 at gene, mRNA and protein levels are known to be associated with a range of cancers: leukemia, lymphomas, lung cancer, breast cancer, colorectal cancer, gastric cancer, hepatocellular cancer and so on. Accumulating evidence indicates that SUV39H2 acts as an oncogene and contributes to the initiation and progression of cancers. |
Other Clones
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Unconjugated
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