Recombinant SV2A Monoclonal Antibody (AN301990L)

Name: Recombinant SV2A Monoclonal Antibody
Brand: Elabscience
SKU: AN301990L
Price: 249.0 USD
Availability: InStock

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Recombinant SV2A Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: Mouse brain,?Rat brain Verified Samples in IHC: Human cerebrum, Human pancreas, Human placenta (negative tissue), Mouse cerebrum, Rat cerebrum
Dilution	WB 1:1000, IHC 1:500-1:1000
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant fragment
Abbre	SV2A
Synonyms	KIAA, PSEC, SV2, SV2A, KIAA0736, PSEC0174
Swissprot	Q7L0J3
Calculated MW	83 kDa
Observed MW	100 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Neuroscience
Clone No.	A710
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	SV2s are a family of synaptic vesicle proteins expressed in both neurons and endocrine cells. SV2s function in the regulation of synaptic vesicle traffic, cytoplasmic Ca2+ levels in the nerve terminal during repetitive stimulation and the facilitation of synaptic transmission. There are three isoforms of SV2: SV2A, SV2B and SV2C. Each of these isoforms are structured similarly but expressed varyingly. SV2C, a minor isoform of SV2, expressed in a small subset of neurons located within the basal forebrain, midbrain and brainstem. SV2B, a major isoform of SV2 is expressed more abundantly, although rarely without the coexpression of SV2A. SV2A, the other major isoform of SV2 is the most widely expressed. SV2A is located in the presynaptic nerve terminals of almost every neuron throughout the nervous system. In addition, it is also located in most neuroendocrine secretory granules. SV2A has been identified as a critical protein for proper function of the central nervous system and has been linked to the physiopathology of epilepsy. In addition to the epileptic affects of this protein, mutations in it have also been seen to result in schizophrenia.