Recombinant TMS1/ASC Monoclonal Antibody (AN301903L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat (negative control), THP-1, HL-60, HeLa (negative control) Verified Samples in IHC: Human breast cancer, Human colon cancer, Human tonsil Verified Samples in IF: THP-1 |
| Dilution | WB 1:1000, IHC 1:100-1:500, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human TMS1/ASC fragment |
| Abbre | TMS1/ASC |
| Synonyms | CARD, ASC / TMS, PYCARD, ASC, CARD5, TMS, TMS-1, TMS1, PYD and CARD domain containing, ASC / TMS1, Apoptosis associated speck like protein containing a CARD, Apoptosis-associated speck-like protein containing a CARD, CARD 5, Caspase recruitment domain containing protein 5, Caspase recruitment domain protein 5, Caspase recruitment domain-containing protein 5, hASC, MGC10332, PYD and CARD domain containing protein, PYD and CARD domain-containing protein, Target of methylation induced silencing 1, Target of methylation-induced silencing 1, TMS 1 |
| Swissprot | |
| Calculated MW | 22, 15 kDa |
| Observed MW |
22, 15 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Mitochondrion, Endoplasmic reticulum, Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Cancer |
| Clone No. | A619 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Target of methylation-induced silencing (TMS1)/Apoptosis-associated speck-like protein containing a CARD (ASC), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The ASC/TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells, and has since been found to be silenced in a number of other cancers, including ovarian cancer, glioblastoma, melanoma, gastric cancer, lung cancer, and prostate cancer. Expression of ASC/TMS1 can be induced by pro-apoptotic/inflammatory stimuli. During apoptosis ASC/TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. ASC/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals. |
Other Clones
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